Genetic Diversity Among Puntius Sophore Complex Using Restriction Fragment Length Polymorphism
Abstract
Restriction Fragment Length Polymorphism (RFLP) analysis of genomic DNA was employed to characterize species of
Puntius sophore complex, a benthopelagic freshwater fish species. Samples of five species of Puntius viz, Puntius sophore, Puntius
amphibius, Puntius chola, Puntius bimaculatus and Puntius dorsalis from the east flowing Tamiraparani and Kuzhithurai and west flowing
Tamiraparani river basins (Western Ghats) were analyzed. The restriction enzyme employed (Hind III) generated species-specific DNA
patterns for each of the five species. DNA exhibited considerable polymorphism among species. All samples showed relatively high
values of diversity. The relation between species is discussed.
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