The Use of Serological Tests (I.F.A.T. and iELISA) for Evaluation of Specific Antibodies Level in Vaccinated and Nonvaccinated Animals Against Anthrax

Abstract

Various diseases, especially infectious ones, have caused untold harm to humanity in centuries. Among these, special attention should be paid to infectious diseases, especially anthrax, which reduces a lot the number of heads and livestock products. One of the most important tools to combat this infection are vaccinations. Today there are different kinds of vaccines against anthrax. The aim of this study was the use of IFA tests and iELISA to determine the efficacy of vaccines against anthrax. For the implementation of the IFA test bacterial emulsions prepared with bacterial strains of B. anthracis Stern 34F2 and Pasteur were used as antigens. As for determining the level of antibodies in the serum by iELISA was used Anthrax Protective antigen IgG ELISA kit. Sera of sheep and goat vaccinated with capsular and noncapsular vaccines were analyzed with both methods 21 days, 9 months and 12 months after vaccination. The results obtained show that sheep respond better to the vaccine than goat. The index of IgG anti-PA antibodies between vaccines, determined by ELISA, varies from 3.3 to 1.6 and 3.85 to 1.94, respectively, in the sera of animals vaccinated with noncapsular and capsular vaccines 21 days and 12 months after vaccination. For evaluation of vaccine immunogenicity, techniques of IgG anti-PA ELISA and indirect immunofluorescence (IFAT) can be used as alternative methods of control by the State Laboratory of Control of Veterinary Bioproducts.

DOI: 10.5901/ajis.2013.v2n9p642